Hydroxylation of Testosterone at Carbons 1, 2, 6, 7, 15 and 16 by the Hepatic Microsomal Fraction from Adult Female C57BL/6J Mice

Abstract

The metabolism of a mixture of [4-¹⁴C]- and [7β-²H]testosterone by the hepatic microsomal fraction from adult female C57BL/6J mice has been investigated. The following metabolites were identified by their mass spectra and by their retention times on gas chromatography on one or two phases: 1ζ-, 2β-, 6α-, 6β-, 7α-, 15α-, 15β-, 16α- and 16β-hydroxytestosterone; 6α-, 6β- and 7α-hydroxy-4-androstene-3,17-dione; and 4-androstene-3,17-dione. A compound tentatively identified as 6- or 7-oxotestosterone was also isolated. 17β-Hydroxy-4,6-androstadien-3-one, 17β-hydroxy-1,4-androstadien-3-one and 4,6-androstadiene-3,17-dione were identified but are considered to arise non-enzymatically from 7α-hydroxytestosterone, 1ζ-hydroxytestosterone and 7α-hydroxy-4-androstene-3,17-dione, respectively.