Long-term organ culture of embryonic rat pancreas in a chemically defined medium.
- 1 January 1976
- journal article
- Vol. 82 (1), 119-28
Abstract
Embryonic rat pancreas anlagen have been grown in a chemically defined medium, subdivided biweekly and recultured for a total time of 10 weeks. The total mass increment during this time was in excess of 1000-fold. Samples were removed for light and electron microscope examination and for periodic measurement of enzymatic activity. Morphogenesis and cytodifferentiation occurred, peripheral outgrowth of epithelial buds was followed by the formation of interconnecting tubular structures and, eventually, by the appearance of distinctive acinar cells with zymogen granules. Mitotic figures became conspicuous at the periphery of explants within a day after each subdivision resulting in the formation of new tubular structures and acini. In general, the central area of the explants presented more mature acini with zymogen granules than was manifested at the periphery. The enzymatic activities of amylase, lipase, and chymotrypsin developed maximally during the first week of culture, reached a plateau level by the second week, and remained at a relatively constant level throughout the 10-week culture period.This publication has 15 references indexed in Scilit:
- Mitogenic activity of blood components. I. Thrombin and prothrombin.Proceedings of the National Academy of Sciences, 1975
- Stimulation of Mouse B Lymphocytes by TrypsinThe Journal of Immunology, 1974
- Effects of a partially purified factor from chick embryos on macromolecular synthesis of embryonic pancreatic epitheliaDevelopmental Biology, 1973
- The effect of hydrocortisone on exportable enzyme accumulation in the differentiating chick pancreasDevelopmental Biology, 1972
- Assay of nanogram levels of triglyceride lipase with a radioactive substrateJournal of Lipid Research, 1972
- Release from Density Dependent Growth Inhibition by Proteolytic EnzymesNature, 1970
- A Rapid Saccharogenic Method for the Determination of Serum AmylaseClinical Chemistry, 1965
- THE USE OF LEAD CITRATE AT HIGH pH AS AN ELECTRON-OPAQUE STAIN IN ELECTRON MICROSCOPYThe Journal of cell biology, 1963
- Release of lysosomal protease by ultraviolet irradiation and inhibition by hydrocortisoneExperimental Cell Research, 1961
- PROTEIN MEASUREMENT WITH THE FOLIN PHENOL REAGENTJournal of Biological Chemistry, 1951