The molecular selectivity of phospholipase D in HL60 granulocytes
- 15 May 1995
- journal article
- Published by Wiley in FEBS Letters
- Vol. 364 (3), 250-254
- https://doi.org/10.1016/0014-5793(95)00399-t
Abstract
The molecular selectivity of PLD in PMA-stimulated HL60 granulocytes was determined by HPLC analysis of [3H]butanol incorporation into phosphatidyl[3H]butanol (Ptd[3H]But) molecular species. Comparison with phospholipid compositions confirmed that PLD acted primarily on phosphatidylcholine (PtdCho). Apparent enzyme selectivity was suggested by negligible formation of PB16:0/16:0 and preferential synthesis of Ptd[3H]But species containing sn-1 18:0. Culture with exogenous 18:2n−6 or 20:4n−6 readily modified both PtdCho and Ptd[3H]But compositions, and accentuated the apparent selectivity of stimulated PLD for sn-1 18:0 species of PtdCho. Such modifications to PLD-based signalling mechanisms may contribute to the modulatory effects of altered dietary lipid intakes on cellular functionsKeywords
This publication has 17 references indexed in Scilit:
- Phospholipase D and cell signalingCurrent Opinion in Immunology, 1993
- Human neutrophil phospholipase D activation by N-formylmethionyl-leucylphenylalanine reveals a two-step process for the control of phosphatidylcholine breakdown and oxidative burstBiochemical Journal, 1992
- Long-term supplementation with n-3 fatty acids, II: effect on neutrophil and monocyte chemotaxisScandinavian Journal of Clinical and Laboratory Investigation, 1992
- Quantitative analysis of molecular species of diacylglycerol and phosphatidate formed upon muscarinic receptor activation of human SK-N-SH neuroblastoma cells.Journal of Biological Chemistry, 1991
- A novel and sensitive assay for phospholipase D in intact cellsFEBS Letters, 1990
- A comparison of the specificity of phosphatidylcholine synthesis by human fetal lung maintained in either organ or organotypic cultureBiochemical Journal, 1988
- Method for the sensitive analysis of individual molecular species of phosphatidylcholine by high-performance liquid chromatography using post-column fluorescence detectionJournal of Chromatography B: Biomedical Sciences and Applications, 1987
- Intracellular translocation of phosphatidate phosphohydrolase and its possible role in the control of glycerolipid synthesisProgress in Lipid Research, 1984
- A RAPID METHOD OF TOTAL LIPID EXTRACTION AND PURIFICATIONCanadian Journal of Biochemistry and Physiology, 1959
- A RAPID METHOD OF TOTAL LIPID EXTRACTION AND PURIFICATIONCanadian Journal of Biochemistry and Physiology, 1959