Vesicular transport of cationized ferritin by the epithelium of the rat choroid plexus.
Open Access
- 1 April 1981
- journal article
- Published by Rockefeller University Press in The Journal of cell biology
- Vol. 89 (1), 131-139
- https://doi.org/10.1083/jcb.89.1.131
Abstract
We have studied the transport of ferritin that was internalized by coated micropinocytic vesicles at the apical surface of the choroid plexus epithelium in situ. After ventriculocisternal perfusion of native ferritin (NF) or cationized ferritin (CF), three routes followed by the tracers are revealed: (a) to lysosomes, (b) to cisternal compartments, and (c) to the basolateral cell surface. (a) NF is micropinocytosed to a very limited degree and appears in a few lysosomal elements whereas CF is taken up in large amounts and can be followed, via endocytic vacuoles and light multivesicular bodies, to dark multivesicular bodies and dense bodies. (b) Occasionally, CF particles are found in cisterns that may represent GERL or trans-Golgi elements, whereas stacked Golgi cisterns never contain CF. (c) Transepithelial vesicular transport of CF is distinctly revealed. The intercellular spaces of the epithelium, below the apical tight junctions, contain numerous clusters of CF particles, often associated with surface-connected, coated vesicles. Vesicles in the process of exocytosis of CF are also present at the basal epithelial surface, whereas connective tissue elements below the epithelium are unlabeled. Our conclusion is that fluid and solutes removed from the cerebrospinal fluid by endocytosis either become sequestered in the lysosomal apparatus of the choroidal epithelium or are transported to the basolateral surface. However, our results do not indicate any significant recycling via Golgi complexes of internalized apical cell membrane.Keywords
This publication has 26 references indexed in Scilit:
- Regeneration of plasmalemma and surface properties in macrophagesExperimental Cell Research, 1976
- The distribution and mobility of anionic sites on the surfaces of baby hamster kidney cells.The Journal of cell biology, 1975
- Concordance between morphological and biochemical estimates of fluid spaces in rat brain cortex slicesExperimental Brain Research, 1974
- Brain specific synaptosomal membrane proteins demonstrated by crossed immunoelectrophoresisJournal of Neurochemistry, 1974
- LOCALIZATION OF SODIUM PUMPS IN THE CHOROID PLEXUS EPITHELIUMThe Journal of cell biology, 1973
- Mechanisms of ion transport across the choroid plexusThe Journal of Physiology, 1972
- Use of cationized ferritin as a label of negative charges on cell surfacesJournal of Ultrastructure Research, 1972
- Evidence for Choroid-Plexus Arsorption in HydrocephalusNew England Journal of Medicine, 1970
- Permeability of the choroid plexus of the rabbit to several solutesAmerican Journal of Physiology-Legacy Content, 1966
- Observations on the transport of proteins by the isolated choroid plexusActa Neuropathologica, 1964