Characterization of a temperature-sensitive mutant of human adenovirus type 7
- 28 February 1977
- journal article
- research article
- Published by American Society for Microbiology in Journal of Virology
- Vol. 21 (3), 1159-1169
- https://doi.org/10.1128/jvi.21.3.1159-1169.1977
Abstract
The properties of a naturally occurring temperature-sensitive (ts) mutant of human adenovirus type 7 (Ad7) were studied. Mutant Ad7 (19) or E46- was the nonhybrid adenovirus component derived from the defective SV-40-Ad7 hybrid (PARA). Growth of the mutant was restricted at 40.5.degree. C, and the ratios of virus yields in [human oral carcinoma] KB cells at 40.5.degree. and 33.degree. C were 10-2 to 10-3. Viral DNA synthesis and the synthesis of adenovirus-specific antigens (tumor, capsid, hexon and penton antigens) appeared normal at the restrictive temperature. The assembly of virus particles was aberrant, as determined by thin-section of infected cells. The infectivity of mutant virions was heat labile at 50.degree. C, suggesting a ts defect in a structural component of the virion. Analysis by polyacrylamide gel electrophoresis of [35S]methionine-labeled polypeptides synthesized in mutant-infected cells suggested that at least the major virion polypeptides were synthesized at the restrictive temperature. A lack of inhibition of host protein synthesis late in mutant infections, as compared with wild-type (WT) infections at both the permissive and nonpermissive temperatures, made quantitation of infected-cell polypeptides difficult. Analysis of the assembly of capsomeres from cytoplasmic extracts of infected cells on sucrose gradients and by non-dissociating polyacrylamide gel electrophoresis suggested that hexon capsomeres were made at 40.5.degree. C. The hexon capsomeres made by the mutant at 33.degree. or 40.5.degree. C displayed a decreased migration in the non-dissociating gels compared with the WT hexon capsomeres. The molecular weights of the mutant and WT hexon polypeptides were identical. The ts lesion of this group B human Ad7 mutant may be reflected in altered hexons. The mutant Ad7 interfered with the replication of adenovirus types 2 and 21 at the elevated temperature.This publication has 42 references indexed in Scilit:
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