Genomic Location and Lack of Phosphorylation of the HSV Immediate-Early Polypeptide IE 12

Abstract
Herpes simplex virus type 1 (HSV-1) induces an immediate-early (IE) polypeptide IE 12. An equivalent polypeptide coded by HSV-2 which migrated slightly more slowly on sodium dodecyl sulfate polyacrylamide gels was identified and designated IE 12.3. Analysis of the serotype of the IE polypeptide induced by 5 HSV-1/HSV-2 intertypic recombinants when correlated with their genome structures showed that IE 12/IE 12.3 mapped in the region spanning the short terminal repeat/short unique region junction. Unlike 4 other IE polypeptides induced by HSV-1 (IEs 175, 110, 68 and 63), IE 12 was not detectably phosphorylated.