A cell autonomous function of Brachyury in T/T embryonic stem cell chimaeras
- 1 September 1991
- journal article
- Published by Springer Nature in Nature
- Vol. 353 (6342), 348-351
- https://doi.org/10.1038/353348a0
Abstract
Developmental genetics has shown that the Brachyury (T) gene has a key role in mesoderm formation during gastrulation in the mouse. Homozygous embryos have a defective allantois, degenerate or absent notochord and disrupted primitive streak and node. The neural tube is kinked and somite formation interrupted. The T gene has been cloned and is expressed during the early stages of gastrulation, being restricted to the primitive streak region, nascent mesoderm and notochord. Neither the sequence of the gene nor its expression pattern define its developmental function. To study the cell autonomy of the T mutation we have isolated and genetically characterized embryonic stem cell lines and studied their behaviour in chimaeras. T/+ embryonic stem cells form normal chimaeras, whereas T/T in equilibrium with +/+ chimaeras mimic the T/T mutant phenotype. The results indicate that the T gene acts cell autonomously in the primitive streak and notochord but may activate a signalling pathway involved in the specification of other mesodermal tissues.Keywords
This publication has 19 references indexed in Scilit:
- Expression pattern of the mouse T gene and its role in mesoderm formationNature, 1990
- Cloning of the T gene required in mesoderm formation in the mouseNature, 1990
- The mouse Brachyury gene and mesoderm formationTrends in Genetics, 1990
- Defects in the archenteron of mouse embryos homozygous for the T–mutationDifferentiation, 1984
- Effects of the Brachyury (T) mutation on morphogenetic movement in the mouse embryoDevelopmental Biology, 1981
- Rescue of a lethal T/t locus genotype by chimaerism with normal embryosNature, 1978
- Curtailed, a new dominant T-allele in the house mouseGenetics Research, 1966
- A NEW DOMINANT T-ALLELE IN THE HOUSE MOUSEJournal of Heredity, 1959
- The Development of Normal and Homozygous Brachy (T/T) Mouse Embryos in the Extraembryonic Coelom of the ChickProceedings of the National Academy of Sciences, 1944
- Development of the short‐tailed mutant in the house mouseJournal of Experimental Zoology, 1935