CHROMATOGRAPHIC PURIFICATION OF HUMAN SERUM ACCELERATOR GLOBULIN 1

Abstract

A method is described for the purification of human serum accelerator globulin by chromatography on Amberlite IR-400 columns. The technique involves the prior isolation of a crude plasma accelerator globulin preparation from freshly-collected, human, ACD plasma by barium citrate adsorption and acid precipitation. The material thus prepared is then activated to serum accelerator globulin followed by isolation on a chromatographic column. Although the products thus prepared are not chemically homogeneous, they have the following properties: (1) An UV spectrum similar to a protein-peptide containing an aromatic amino acid; (2) A mobility between that of a beta and gamma globulin as measured by filter paper electrophoresis. (3) Loss of activity upon dialysis which may be due to destruction or absorption; (4) Marked instability which can be somewhat minimized in the presence of 50% glycerol.