An examination of factors affecting the efficiency ofAgrobacterium-mediated transformation of tomato
- 1 December 1996
- journal article
- Published by Springer Nature in Plant Cell Reports
- Vol. 16 (3-4), 235-240
- https://doi.org/10.1007/bf01890875
Abstract
An improved protocol forAgrobacterium-mediated transformation of the tomato cultivar Moneymaker was developed by examining the effects of six different factors on the efficiency of transformation. Explant size, explant orientation, gelling agent and plate sealant were found to affect transformation efficiency. Two other factors, type of explant (hypocotyl or cotyledon) and frequency of transfer to fresh selective regeneration medium, did not have any effect on transformation efficiency. By combining the best treatments for each factor, an average transformation efficiency of 10.6% was obtained for Moneymaker.Keywords
This publication has 11 references indexed in Scilit:
- Microprep protocol for extraction of DNA from tomato and other herbaceous plantsPlant Molecular Biology Reporter, 1995
- Re-evaluation of Conditions for Plant Regeneration and Agrobacterium-Mediated Transformation from Tomato (Lycopersicon esculentum)Journal of Experimental Botany, 1993
- Factors influencing transformation frequency of tomato (Lycopersicon esculentum)Plant Cell Reports, 1993
- Effects of tomato cultivar, leaf age, and bacterial strain on transformation by Agrobacterium tumefaciensPlant Cell, Tissue and Organ Culture (PCTOC), 1991
- The complete sequence of soybean chlorotic mottle virus DNA and the identification of a novel promoterNucleic Acids Research, 1989
- Transformation of cultivated tomato by a binary vector in Agrobacterium rhizogenes: transgenic plants with normal phenotypes harbor binary vector T-DNA, but no Ri-plasmid T-DNATheoretical and Applied Genetics, 1986
- Leaf disc transformation of cultivated tomato (L. esculentum) using Agrobacterium tumefaciensPlant Cell Reports, 1986
- Genomic sequencing.Proceedings of the National Academy of Sciences, 1984
- Efficient hybridization between Lycopersicon esculentum and L. peruvianum via embryo callusTheoretical and Applied Genetics, 1981
- A Revised Medium for Rapid Growth and Bio Assays with Tobacco Tissue CulturesPhysiologia Plantarum, 1962