Electron microscopy of frozen-hydrated biological material
- 20 February 1986
- journal article
- review article
- Published by Springer Nature in Nature
- Vol. 319 (6055), 631-636
- https://doi.org/10.1038/319631a0
Abstract
The electron microscopy of frozen-hydrated specimens has the potential to allow biological material to be examined in conditions very similar to the native state, and so has excited considerable interest. Although not all of the problems associated with the method have been solved, the technique has already provided important insights into macromolecular structure and assembly and promises to be a powerful tool for investigating the structure of cellular components and their interactions.Keywords
This publication has 48 references indexed in Scilit:
- Cryo‐electron microscopy and three‐dimensional reconstruction of actin filamentsJournal of Microscopy, 1986
- Cryo‐electron microscopy of helical particles TMV and T4 polyheadsJournal of Microscopy, 1985
- Cryo‐electron microscopy of tropomyosin magnesium paracrystalsJournal of Microscopy, 1985
- Freezing, sectioning and observation artefacts of frozen hydrated sections for electron microscopyJournal of Microscopy, 1983
- Electron microscopy of frozen hydrated sections of vitreous ice and vitrified biological samplesJournal of Microscopy, 1983
- Electron microscopy of frozen biological suspensionsJournal of Microscopy, 1983
- Electron microscopy of frozen water and aqueous solutionsJournal of Microscopy, 1982
- VITRIFICATION OF PURE WATER FOR ELECTRON MICROSCOPYJournal of Microscopy, 1981
- Mass loss and etching of frozen hydrated specimensJournal of Microscopy, 1979
- Radiation damage relative to transmission electron microscopy of biological specimens at low temperature: a reviewJournal of Microscopy, 1978