Abstract
When the parasitic dipteron Pseudosarcophaga affinis (Fall.) was reared aseptically on chemically defined media; thiamine, riboflavin, calcium pantothenate, nicotinic acid, choline chloride, and biotin were essential for larval growth and development, but not vitamin B12, pyridoxine, folic acid, para-aminobenzoic acid, or inositol. Vitamin B12had a slight beneficial effect on pupation, whereas the omission of para-aminobenzoic acid had a slight stimulatory effect on growth and development. The responses of larvae to deficiencies of different vitamins reached a decisive point at different stages of larval development.
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