Complete nucleotide sequence of a cloned chickena-globin cDNA
Open Access
- 1 January 1980
- journal article
- research article
- Published by Oxford University Press (OUP) in Nucleic Acids Research
- Vol. 8 (6), 1187-1200
- https://doi.org/10.1093/nar/8.6.1187
Abstract
Chicken globin double-stranded cDNA was synthesised from anaemic adult reticulocyte α- and β-globin mPNA and ligated into the Hind III site of pBR322 using synthetic Hind III decamers. Transformation of E. coli ×1776 resulted in the production of a number of α- and β-cDNA clones. One of the α-type clones. (pCGα-8) was fully sequenced and found to code for neither αA-nor αD-globin. Partial sequencing of the other α-cDNA clones indicates that they are all of the same type.Keywords
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