Fidelity of DNA Polymerase‐β in Neurons from Young and Very Aged Mice
- 5 October 1985
- journal article
- research article
- Published by Wiley in Journal of Neurochemistry
- Vol. 45 (4), 1273-1278
- https://doi.org/10.1111/j.1471-4159.1985.tb05553.x
Abstract
Neurons do not divide during adult life and thus they provide a unique system to study the effects of age‐accumulated damage to DNA in the absence of DNA replication. We have analyzed DNA polymerase activity in neurons isolated from young adult and very aged mice. The predominant catalytic activity is DNA polymerase‐β and it is present in similar amounts in neurons from young and old mice. This polymerase is highly errorprone in copying φX174 DNA, the error frequency being about 1/7,000 and not significantly different when obtained from young and old animals. This high infidelity is considered with respect to DNA repair and the protein synthesis error catastrophe theory of aging.Keywords
This publication has 27 references indexed in Scilit:
- DNA-polymerases in neuron and glial cells of developing and aging mouse brainJournal of Neuroscience Research, 1983
- Procedure for isolation of neuron‐ and astrocyte‐enriched fractions from chick brain of different agesJournal of Neuroscience Research, 1983
- Fidelity of Mammalian DNA PolymerasesScience, 1981
- On the activity and fidelity of chromatin‐associated hepatic DNA polymerase‐β in aging murine species of different life spansJournal of Cellular Physiology, 1981
- Increased error frequency of DNA polymerases from senescent human fibroblastsJournal of Molecular Biology, 1981
- On the fidelity of deoxyribonucleic acid synthesis directed by chromatin-associated deoxyribonucleic acid polymerase .beta.Biochemistry, 1980
- The development of the septal region in the rat. I. Neurogenesis examined with 3H‐thymidine autoradiographyJournal of Comparative Neurology, 1979
- The use of thin acrylamide gels for DNA sequencingFEBS Letters, 1978
- Nucleotide and amino acid sequences of gene G of φX174Journal of Molecular Biology, 1976
- THE RELATIONSHIP BETWEEN DNA SYNTHESIS AND THE SYNTHESIS OF NUCLEAR PROTEINS IN RAT BRAIN DURING DEVELOPMENT1Journal of Neurochemistry, 1972