GC-MS Derivatization Studies: The Formation of Dexamethasone MO-TMS
- 1 January 1972
- journal article
- research article
- Published by Taylor & Francis in Analytical Letters
- Vol. 5 (12), 905-913
- https://doi.org/10.1080/00032717208062155
Abstract
The 16α-methyl group in dexamethasone increases drastically the steric hindrance to reaction of both the 20-ketone and 17α-hydroxyl groups, as shown by kinetic studies with GC-MS techniques. A procedure is described for the preparation of the MO derivative (reaction et 60[ddot]C for 3 hr) and complete conversion of all hydroxyl groups to TMS ether groups (reaction at 100[ddot]C for 6 hr). The resulting MO-TMS derivative is thermostable and suitable for use in GC-MS methods. The procedures usually employed in urinary steroid studies are satisfactory for prednisone and prednisolone.Keywords
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