An assay procedure for a succinate–neotetrazolium-reductase system
- 1 March 1960
- journal article
- research article
- Published by Portland Press Ltd. in Biochemical Journal
- Vol. 74 (3), 591-596
- https://doi.org/10.1042/bj0740591
Abstract
A method is described for estimating an enzyme system (succinate-neotetrazolium reductase) coupling the oxidation of succinate with the reduction of neotetrazolium chloride. In the presence of added vitamin K3 (1 m[image]) and vitamin C (approximately 1 m[image]) the production of formazan increases proportionately with the amount of tissue added. Assay conditions are described for liver homogenates applicable to 0-15 mg wet weight of liver tissue. The application of the method to intracellular fractions and to other tissues is discussed.Keywords
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