Separation of Thiamin, Thiamin Antagonists and Their Phosphate Esters by High-Performance Liquid Chromatography
- 1 January 1980
- journal article
- research article
- Published by Informa UK Limited in Journal of Liquid Chromatography
- Vol. 3 (11), 1697-1712
- https://doi.org/10.1080/01483918008064761
Abstract
Methods are presented for the separation of the phosphate esters of thiamine, oxythiamine or pyrithiamine and the separation of thiamine and oxythiamine by high-performance liquid chromatography. Detection by means of a UV monitor and fluorometer with a post-column oxidation system is described which allows for selective detection of thiochrome, pyrichrome and their respective phosphate esters. Anion exchange and ion-pair reverse phase chromatography are the chromatographic modes of separation.This publication has 20 references indexed in Scilit:
- Analysis of thiamine and its phosphate esters by high-performamze liquid chromatographyAnalytical Biochemistry, 1979
- Gas chromatography of thiamine and derivativesJournal of Chromatography A, 1973
- Quantitative determination of thiamine and its phosphate esters by sephadex gel filtrationBiochimica et Biophysica Acta (BBA) - General Subjects, 1972
- Gas chromatography-mass spectrometry of the trimethylsilyl derivatives of various thiamine metabolitesAnalytical Biochemistry, 1970
- Microassay of thiamine and its phosphate esters after separation by paper chromatographyAnalytical Biochemistry, 1966
- Über die Trennung der Phosphorsäureester von Thiamin und seinen Analogen durch GradientenelutionHoppe-Seyler´s Zeitschrift Für Physiologische Chemie, 1966
- Die Umwandlung des Thiamins in der LeberThe Science of Nature, 1959
- Chromatographic separation and determination of thiamine and thiamine phosphoric esters on ion-exchange resinsJournal of Chromatography A, 1958
- Separation and quantitative determination of thiamine and thiamine phosphoric esters and their preparation in pure formBiochimica et Biophysica Acta, 1954
- On the Presence of the Triphosphothiamine (TPT) in the LiverScience, 1952