Abstract
Sexual isolation between the closely related spp., D. persimilis and D. pseudoobscura, was studied by means of male multiple "choice" tests, female multiple "choice" tests, and multiple "choice" expts. involving the males and females of both spp. The female "choice" and multiple "choice" expts, gave essentially the same result, a much higher degree of sexual isolation (I=0.069 and I=0.058), than the male "choice" expts. (I=0.457). These results indicate that the high degree of sexual isolation between these 2 spp. is primarily due to the behavior of the females.