Application to Mammalian Tissues of the Chemiluminescent Method for Detecting Acetylcholine
- 1 July 1982
- journal article
- Published by Wiley in Journal of Neurochemistry
- Vol. 39 (1), 248-250
- https://doi.org/10.1111/j.1471-4159.1982.tb04727.x
Abstract
It is now possible to extend to mammalian tissues the chemilumi-nescent acetylcholine assay. Mammalian tissue extracts must be treated with oxidants (which is not necessary for electric organ extracts). The assay can then be performed as previously described (acetylcholinesterase hydrolyses acetylcholine; choline oxidase converts choline to betaine and H2O2, which gives off light in the presence of luminol and peroxidase). It is also shown that release experiments can be performed on mammalian tissue slices (mouse caudate nucleus) after the slice is washed in oxygenated saline solutions.Keywords
This publication has 3 references indexed in Scilit:
- Continuous Determination by a Chemiluminescent Method of Acetylcholine Release and Compartmentation in Torpedo Electric Organ SynaptosomesJournal of Neurochemistry, 1981
- Chemiluminescent determination of acetylcholine, and continuous detection of its release from torpedo electric organ synapses and synaptosomesNeurochemistry International, 1981
- A METHOD FOR DETERMINATION OF ACETYLCHOLINE BY SLOW PYROLYSIS COMBINED WITH MASS FRAGMENTOGRAPHY ON A PACKED CAPILLARY COLUMNJournal of Neurochemistry, 1979