Lipid Synthesis and Ultrastructure of Isolated Barley Chloroplasts
- 1 February 1968
- journal article
- research article
- Published by Oxford University Press (OUP) in Plant Physiology
- Vol. 43 (2), 163-187
- https://doi.org/10.1104/pp.43.2.163
Abstract
The cell organelle contents of chloro-plast preparations made from barley leaves with salt and sucrose isolation media at pH 6 and 8 were determined and compared with the acetate incorporating activity of these preparations. A chloroplast preparation obtained with 0.5[image] sucrose at pH 8 gave the highest number of intact chloroplasts (with envelope and stroma), the lowest number of contaminating mitochondria, and the highest activity in light dependent acetate incorporation into lipids. In the preparations observed, the light induced lipid synthesizing capacity correlates well with the percentage of intact chloroplasts. It is suggested that the intact chloroplasts are responsible for the light induced lipid synthesis of the preparations and that the synthesizing enzymes are localized in the chloroplast stroma. Acetate is mainly incorporated into palmitic and oleic acids. The low yield of intact chloroplasts and of light induced lipid synthesis in preparations isolated at pH 6 seem to result from the action of galactolipid lipase(s).This publication has 16 references indexed in Scilit:
- Correlation between Photosynthetic Activity and Membrane Integrity in Isolated Pea ChloroplastsPlant Physiology, 1965
- Chloroplast Integrity and Biochemical FunctionPlant Physiology, 1965
- Relationship of the Syntheses of Lipid and Water Soluble Acids by Chloroplast PreparationsPlant Physiology, 1965
- THE FINE STRUCTURE OF CHLOROPLAST STROMA FOLLOWING ALDEHYDE OSMIUM-TETROXIDE FIXATIONThe Journal of cell biology, 1965
- Hydrolysis of Monogalactosyl and Digalactosyl Diglycerides by Specific Enzymes in Runner-Bean Leaves*Biochemistry, 1964
- Fat metabolism in higher plants XX. relation of fatty acid synthesis and photophosphorylation in lettuce chloroplastsBiochimica et Biophysica Acta, 1963
- The biosynthesis of long-chain fatty acids by lettuce chloroplast preparationsBiochimica et Biophysica Acta, 1963
- A RAPID METHOD OF TOTAL LIPID EXTRACTION AND PURIFICATIONCanadian Journal of Biochemistry and Physiology, 1959
- Triphosphopyridine Nucleotide as a Catalyst of Photosynthetic PhosphorylationNature, 1957
- Chlorophyll-letale und der submikroskopische Formwechsel der PlastidenExperimental Cell Research, 1957