The Effects of U.V.-light, Ionizing Radiation and the Carcinogen N-acetoxy-2-fluorenylacetamide on the Developmentin Vitroof One- and Two-cell Mouse Embryos
- 1 January 1976
- journal article
- Published by Taylor & Francis in International Journal of Radiation Biology and Related Studies in Physics, Chemistry and Medicine
- Vol. 30 (5), 401-408
- https://doi.org/10.1080/09553007614551221
Abstract
The development of one- and two-cell mouse embryos to morula-blastula stages was followed in vitro after treatment with low doses of U.V.-light, ionizing radiation or N-acetoxy-2-fluorenylacetamide. Exposure of one-cell embryos to either radiation source 18 and 24 hours after human chorionic gonadotropin injections prevented maturation, most embryos being arrested at the one-cell stage and a few at the two-cell stage. Two-cell embryos, however, were not sensitive to low doses of either U.V. or X-irradiation and developed normally. Treatment of early one-cell embryos with the carcinogen, N-acetoxy-2-fluorenyl-acetamide (0-7 muM), also arrested development, whereas exposure of late one-cell embryos did not completely prevent maturation to morula-blastula stages. Exposure of two-cell embryos to the same concentration of carcinogen had no effect on their development to blastulas. Results with all three agents showed that mouse embryos at the one-cell stage are more sensitive than those at the two-cell stage, as judged by their ability to develop in vitro.Keywords
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