Dependency on Acid-base Status of Blood of Oxyhemoglobin Dissociation and 2, 3-Diphosphoglycerate Level in Human Erythrocytes: I. In Vitro Studies on Reduced and Oxygenated Blood

Abstract

The intraerythrocytic 2,3-diphosphoglycerate concentration decreases with decreasing pH below 7.40. Oxyhemoglobin dissociation is thereby changed, the oxygen affinity being increased. This effect counteracts the Bohr-effect, also quantitatively, leading to a nearly constant oxygen affinity at low pH values. The effect of anaerobic incubation on the 2,3-diphosphoglycerate level could be explained in terms of changes in intraerythrocytic pH caused by the deoxygenation of hemoglobin.