Laser Raman spectroscopy of the lens in situ, measured in an anesthetized rabbit
- 1 January 1981
- journal article
- Published by Taylor & Francis in Current Eye Research
- Vol. 1 (10), 615-618
- https://doi.org/10.3109/02713688109001863
Abstract
We have obtained the first Raman spectrum from the lens of a live animal. A laser beam (514.5 nm; 15 mW) was directed into the eye of an anesthetized rabbit at 60° from the visual axis and Raman emission was collected at 90° from the incident beam. The power density at the retina was estimated at 0.5 W/cm2. The entire scattering column in the lens can be imaged on the entrance slit of a spectrometer with so little distortion that Raman “optical dissection” analysis (Askren, Yu and Kuck (1979) Exp. Eye Res. 29_, 647) can be performed on the in situ lens. The advantages of multichannel detectors over photomultiplier tubes with respect to in situ measurements are discussed.Keywords
This publication has 5 references indexed in Scilit:
- The molecular structure and stability of the eye lens: X-ray analysis of γ-crystallin IINature, 1981
- Variation of the concentration of sulfhydryl along the visual axis of aging lenses by laser Raman optical dissection techniqueExperimental Eye Research, 1979
- Raman spectra of bird and reptile lens proteinsExperimental Eye Research, 1977
- Prevalence of α-helical form in avian lens proteinsExperimental Eye Research, 1976
- Single-crystal raman spectra of native insulinArchives of Biochemistry and Biophysics, 1974