Non-Steady-State Kinetics for a Double-Intermediate Enzyme Mechanism: The Case of High Enzyme Concentrations

Abstract

An equation has been derived for the non-steady-state kinetics arising from the mechanism[Formula: see text]in which the concentration of enzyme E is greatly in excess of that of the substrate A; EA and EA′ are two intermediates and X and Y the products. Solutions for the rate of formation of the first product X were given by Kasserra and Laidler. The present work is concerned with the rate of formation of Y. A general equation is derived for the rate of production of Y, and it is shown that under certain simplifying conditions, easily realized in practice, the rate law reduces to [Formula: see text] study of the rate of formation of Y therefore leads to a determination of k3.