Deoxyribonucleic acid relationships among marine vibrios pathogenic to fish
- 1 August 1977
- journal article
- research article
- Published by Canadian Science Publishing in Canadian Journal of Microbiology
- Vol. 23 (8), 954-958
- https://doi.org/10.1139/m77-142
Abstract
Polynucleotide sequence relationships among 19 strains of marine vibrios, 15 of which were pathogenic to fish, were assessed by analysis of DNA–DNA homo- and hetero-duplexes with the endonuclease S1. DNA base compositions (mole% guanine–cytosine (GC) of selected vibrio strains were determined by thermal denaturation. Pathogenic strains from the Pacific Northwest had identical mole % GC but could be divided into two discrete, yet related, DNA-homology groups. One highly related group was typical of Vibrio anguillarum and showed greater than 70% within-group polynucleotide sequence homology. Second group (designated V1669) showed greater than 83% within-group homology but only about 60% relatedness to V. anguillarum. Neither group showed a significant degree of relatedness to V. parahaemolyticus (biotypes parahaemolyticus or alginolyticus) or to representative strains of any of the other previously described homology groups which we tested.This publication has 1 reference indexed in Scilit:
- Determination of the base composition of deoxyribonucleic acid from its thermal denaturation temperatureJournal of Molecular Biology, 1962