Production of bovine tetrapolid embryos by electrofusion and their developmental capability in vitro
- 1 November 1989
- journal article
- research article
- Published by Wiley in Gamete Research
- Vol. 24 (3), 261-267
- https://doi.org/10.1002/mrd.1120240303
Abstract
Optimal conditions of electrofusion for blastomeres of two‐cell bovine embryos to produce tetraploid embryos were investigated. The high fusion rate (73–95%), viability, and develop mental capacity were obtained under a field strength of 1.0 kV/cm with direct current pulses of 10 or 25 μsec duration applied twice. Cytological study showed that 78.6% (11/14 embryos) of embryos exposed to electrofusion had tetraploid chromosome sets and the others were diploid or hexaploid. The tetraploid embryos had the capability to develop up to morulae stage in vitro.Keywords
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