Immunoglobulin determinants on the lymphocytes of normal rabbits. I. Demonstration by the mixed antiglobulin reaction of determinants recognized by anti-gamma, anti-mu, anti-Fab and anti-allotype sera, anti-As4 and anti-As6.

Abstract

Immunoglobulin determinants can be shown on the membrane of circulating lymphocytes of the rabbit by the mixed antiglobulin reaction. An enumeration was made of lymphocytes reacting specifically with anti-γ, anti-μ, anti-Fab and anti-L chain sera. The L chain allotypic determinants As4 and As6 were also shown and reacting cells enumerated. There was a marked dose-dependence between the concentration of the antiglobulin reagent used to treat the lymphocyte suspension and the number of lymphocytes reacting to form rosettes.