Color Test to Measure the Toxicity of Adrenal Cortex Hormones to Lymphocytes.

Abstract

Suspensions of living lymphocytes decolorized the oxidation-reduction indicator, 2,6-dichlorophenol indophenol. This reduction was inhibited by the cytotoxic agents, Lipo-Adrenal Cortex, cortisone, nitrogen mustard and X-rays, but was not affected by desoxycorticosterone. The method was used to titrate Lipo-Adrenal Cortex, the titer being .08 rat units. This color test seemed to be a convenient method for demonstrating and measuring the toxicity of the adrenal cortex hormones to lymphocytes.