Effect of membrane potential on furosemide-inhibitable sodium influxes in human red blood cells
- 1 June 1987
- journal article
- research article
- Published by Springer Nature in The Journal of Membrane Biology
- Vol. 98 (2), 117-124
- https://doi.org/10.1007/bf01872124
Abstract
Furosemide-inhibitable Na influx (a measure of Na/K/Cl cotransport) was determined as a function of membrane potential in human red blood cells. The membrane potential was varied from −42 to +118 mV using valinomycin and gradients of K. The furosemide-inhibitable, unidirectional Na influx was independent of membrane potential over the entire range of potentials. The change in flux per mV, 0.443 μmol/(liter cells·hr· mV), was not significantly different from zero. The mean flux was 153±16μmol/(liter cells·hr) (±sem,n=71). The ouabain and furosemide-resistant influexes of Na and K were also measured as functions of membrane potential using either valinomycin and K or a chloride-free, tartrate flux medium to vary membrane potential. The unidirectional Na influx decreased slightly as the membrane potential was increased from negative potentials to about +10 mV. At higher membrane potentials Na influx rose dramatically with potential. This increase was not reversible and was also observed with K influx.This publication has 22 references indexed in Scilit:
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