Reduction of Ferric Phenanthroline—A Procedure for Determining Serum Uric Acid

Abstract

A method for the determination of uric acid with ferric phenanthroline in a maleic acid buffer at pH 5.5 is presented. The procedure yields accurate (r² = 0.96; recovery 99.3%) and precise (C.V. = 2.08%) results in 5 minutes, usually without deproteinization. The method appears to be more specific for uric acid than the reduction of alkaline phosphotungstate and compares well with uricase determination. Elevated ascorbic acid above 3 mg. per 100 ml. interferes.