A highly sensitive two-photon fluorescent probe for mitochondrial zinc ions in living tissue

Abstract
We report a highly sensitive two-photon probe (SZn2-Mito) which shows a 70-fold two-photon excited fluorescence enhancement in response to Zn2+ and can selectively detect mitochondrial Zn2+ in a rat hippocampal slice at a depth of 100–200 μm by using two-photon microscopy.