DEAE-Cellulose-Urea Chromatography of Casein in the Presence of 2-Mercaptoethanol
Open Access
- 1 July 1966
- journal article
- research article
- Published by American Dairy Science Association in Journal of Dairy Science
- Vol. 49 (7), 792-795
- https://doi.org/10.3168/jds.s0022-0302(66)87947-x
Abstract
K-Casein can be separated from [alpha]sl-and [beta]-casein variants as well as from whole casein by diethyl-aminoethyl (DEAE)-cellulose-urea chromatography after reduction of the disulfide bond(s) by 2-mercaptoethanol. k-Casein in the reduced form elutes from DEAE-cellulose at 0.05 MNaCl. The use of a reduction step simplifies purification of the major casein components (a[alpha] sl -, [beta]) by chromatography.This publication has 10 references indexed in Scilit:
- Simultaneous Phenotyping Procedure for the Principal Proteins of Cow's MilkJournal of Dairy Science, 1965
- Starch-gel electrophoresis of κ-caseinBiochimica et Biophysica Acta (BBA) - General Subjects, 1964
- Polymorphism in κ-casein of cow's milkBiochemical and Biophysical Research Communications, 1964
- Genetic Polymorphism in Caseins of Cow's Milk. IV. Isolation and Properties of β-Caseins A, B, and CJournal of Dairy Science, 1964
- Genetic Polymorphism in Caseins of Cow's Milk. III. Isolation and Properties of αs1-Caseins A, B, and CJournal of Dairy Science, 1964
- Variants of κ-Casein Revealed by Improved Starch Gel ElectrophoresisJournal of Dairy Science, 1964
- Characteristics of kappa-casein in the presence of various dissociating agentsBiochemical and Biophysical Research Communications, 1963
- Fractionnement de la caséine par chromatographie sur colonne de diéthylaminoéthyl-cellulose en milieu uéreBiochimica et Biophysica Acta, 1961
- Analysis of casein fractions by zone electrophoresis in concentrated ureaBiochimica et Biophysica Acta, 1961
- An improved method for the isolation of κ-caseinBiochimica et Biophysica Acta, 1961