Analysis of long range correlations due to coherent light scattering from in-vitro cell arrays using angle-resolved low coherence interferometry
- 1 January 2006
- journal article
- research article
- Published by SPIE-Intl Soc Optical Eng in Journal of Biomedical Optics
- Vol. 11 (3), 034022-034022-7
- https://doi.org/10.1117/1.2209561
Abstract
Angle-resolved low coherence interferometry (a/LCI) enables depth-resolved measurements of scattered light that can be used to recover subsurface structural information, such as the size of cell nuclei. Measurements of nuclear morphology, however, can be complicated by coherent scattering between adjacent cell nuclei. Previous studies have eliminated this component by applying a window filter to Fourier transformed angular data, based on the justification that the coherent scattering must necessarily occur over length scales greater than the cell size. To fully study this effect, results of experiments designed to test the validity of this approach are now presented. The a/LCI technique is used to examine light scattered by regular cell arrays, created using stamped adhesive micropatterned substrates. By varying the array spacing, it is demonstrated that cell-to-cell correlations have a predictable effect on light scattering distributions. These results are compared to image analysis of fluorescence micrographs of the cell array samples. The a/LCI results show that the impact of coherent scattering on nuclear morphology measurements can be eliminated through data filtering.Keywords
This publication has 15 references indexed in Scilit:
- Coherent light scattering by in vitro cell arrays observed with angle-resolved low-coherence interferometryPublished by SPIE-Intl Soc Optical Eng ,2005
- Light Scattering from Intact Cells Reports Oxidative-Stress-Induced Mitochondrial SwellingBiophysical Journal, 2005
- Fabrication of Biofunctionalized Quasi-Three-Dimensional Microstructures of a Nonfouling Comb Polymer Using Soft LithographyAdvanced Functional Materials, 2005
- Prospective grading of neoplastic change in rat esophagus epithelium using angle-resolved low-coherence interferometryJournal of Biomedical Optics, 2005
- Universal Route to Cell Micropatterning Using an Amphiphilic Comb PolymerAdvanced Materials, 2003
- Light scattering from cervical cells throughout neoplastic progression: influence of nuclear morphology, DNA content, and chromatin textureJournal of Biomedical Optics, 2003
- Cellular Organization and Substructure Measured Using Angle-Resolved Low-Coherence InterferometryBiophysical Journal, 2002
- Polarized angular dependent spectroscopy of epithelial cells and epithelial cell nuclei to determine the size scale of scattering structuresJournal of Biomedical Optics, 2002
- Measuring cellular structure at submicrometer scale with light scattering spectroscopyIEEE Journal of Selected Topics in Quantum Electronics, 2001
- 4919 A novel optical biopsy technique using elastic scattering spectroscopy for dysplasia and cancer in barrett's esophagus.Gastrointestinal Endoscopy, 2000