Intramembrane particles and terminal responses following denervation of frog muscle spindles

Abstract

The sizes and population density of intramembrane particles in the non-myelinated sensory nerve terminals of frog muscle spindles were studied in comparison with changes in the responses to spindles to stretch 1–28 days after denervation. The sciatic nerve in the right leg was cut at the dorsal part of the hip joint. Spindle receptors in the left leg were used as controls. Muscle spindles were isolated from semitendinosus and iliofibularis muscles. The mean density of particles, which occurred mainly on the protoplasmic (P) face, declined rapidly to 60% of the control values 2 days after denervation, 30% after 4 days and to less than 10% after 7 days. The mean diameter of particles changed from 8.0 ± 1.5 nm in the control to 12.8 ± 2.5 nm 7 days after denervation. No significant difference between the results for the trunks (> 2 μm in diameter) and the branches (< 0.5 μm) of the non-myelinated axons was observed. The peak rate of afferent discharges during a ramp- and-hold stretch survived up to 4 days after denervation, whereas the static component disappeared after 3 days. It is suggested that the decline to less than 20% of the control density of particles removes the mechanosensitivity of the sensory endings.