Complete nucleotide sequence and properties of the major species of glycine transfer RNA from wheat germ

Abstract

The complete nucleotide sequence of wheat germ tRNA1Gly was determined. It is a small tRNA, containing only 74 nucleotides of which 10 are methylated or otherwise modified. Several important features of this tRNA are: a complete absence of ribothymidine which is the most common methylated nucleoside in tRNA; a methylated sugar in the acceptor stem which is a region almost always free of modified residues; and unusually weak base pairing in the dihydrouridine stem region consisting of only 1 standard A-U base pair in addition to G.PSI., AC and GU. This tRNA functions in a wheat germ cell free protein synthesizing system directed by either natural mRNAs or poly(G,U). The complete nucleotide sequence of wheat germ tRNA1Gly is: pG-C-A-Cm-C-A-G-U-m1G-G-U-C-.PSI.-A-G-D-G-G-U-A-G-A-A-U-A-G-U-A-C-C-C-U-G-C-C-A-m5C-G-G-U-A-C-A-G-A-m5C-m5C-m5C-G-G-G-U-.PSI.-C-G-m1A-U-U-C-C-C-G-G-C-U-G-G-U-G-C-A-C-C-AOH.