Mitotic segregation of cytoplasmic determinants for chloramphenicol resistance in mammalian cells II: Fusions with human cell lines
- 1 January 1977
- journal article
- Published by Springer Nature in Somatic Cell and Molecular Genetics
- Vol. 3 (1), 93-119
- https://doi.org/10.1007/bf01550989
Abstract
Cytoplasmically inherited chloramphenicol (CAP) resistance in human cells has been used to study the interaction between sensitive and resistant mitochondria. Cybrids between two HeLa cells were stable for resistance, grew rapidly and cloned well in CAP, and were O2 tolerant. HeLa-HeLa hybrids were also stable up to 70 doublings in the absence of CAP. Cybrids between HeLa and WI-L2 cells were unstable for resistance for up to 40 doublings, grew slowly and cloned poorly in CAP, and were O2 sensitive (S phase). The growth rate then increased and the cells became stable for resistance, cloned well, and were not O2 sensitive (F phase). Doubling time for S but not F phase cells was proportional to CAP concentration, indicating that both kinds of mitochondria were present and functioning. The instability of CAP resistance in many interstrain but not in intrastrain mouse and human cybrids and hybrids is interpreted in relation to lower eukaryotes.Keywords
This publication has 32 references indexed in Scilit:
- Selective nicking of mammalian mitochondrial DNA in vivo: Photosensitization by incorporation of 5-bromodeoxyuridineJournal of Molecular Biology, 1975
- Repair of pyrimidine dimers in nuclear and mitochondrial DNA of yeast irradiated with low doses of ultraviolet lightJournal of Molecular Biology, 1975
- Chloramphenicol‐resistant mutants of human HeLa cellsFEBS Letters, 1972
- Mitochondrial Genetics in ParameciumNature, 1972
- Evolution of Mixed Populations of Genetically Different Mitochondria in Paramecium aureliaNature, 1972
- Interstrain somatic cell hybrids in the mouse: Chromosome and enzyme analysesExperimental Cell Research, 1970
- HeLa cells resistant to bromodeoxyuridine and deficient in thymidine kinase activityInternational Journal of Cancer, 1966
- Selection of Hybrids from Matings of Fibroblasts in vitro and Their Presumed RecombinantsScience, 1964
- GENETICS OF SOMATIC MAMMALIAN CELLSThe Journal of Experimental Medicine, 1956
- Presence of the Sperm Middle-Piece in the Fertilized Egg of the Mouse (Mus musculus)Nature, 1940