Effect of carbon dioxide on in vitro susceptibility of anaerobic bacteria to erythromycin
Open Access
- 1 December 1981
- journal article
- research article
- Published by American Society for Microbiology in Antimicrobial Agents and Chemotherapy
- Vol. 20 (6), 705-708
- https://doi.org/10.1128/aac.20.6.705
Abstract
The activity of erythromycin against 317 strains of anaerobic bacteria, including 133 strains of the Bacteroides fragilis group, was tested by the agar dilution method in an anaerobic atmosphere with two different concentrations of carbon dioxide and without CO2. The effect of the atmosphere of incubation on the agar surface pH was also determined. All strains grew well in the GasPak (GP) environment. However, 3.5 and 30.3% of strains failed to grow in the 2 and 0% CO2 environments, respectively. The quality of growth was best in the GP environment and poorest in the 0% CO2 environment. Minimal inhibitory concentrations in the GP and 2% CO2 environments were frequently the same or one dilution lower in the 0% than in the GP environment. In the 0% CO2 atmosphere, minimal inhibitory concentrations were usually two to three dilutions lower than in the GP environment. Consequently, only 24% of B. fragilis strains were susceptible to erythromycin in the GP environment, whereas 77% were susceptible in the 0% CO2 environment. For Fusobacterium species, 12% were susceptible to erythromycin in the GP environment, and 73% were susceptible in the 0% CO2 environment. There was a comparable decrease in pH in all three atmospheres tested. In vitro susceptibility testing of erythromycin against anaerobic bacteria should be performed in an atmosphere containing carbon dioxide.This publication has 15 references indexed in Scilit:
- Effect of Carbon Dioxide and pH on Susceptibility of Bacteroides fragilis Group to ErythromycinAntimicrobial Agents and Chemotherapy, 1981
- Susceptibility of Anaerobic Bacteria to Ten Antimicrobial AgentsAntimicrobial Agents and Chemotherapy, 1978
- Susceptibility of Anaerobic Bacteria to 23 Antimicrobial AgentsAntimicrobial Agents and Chemotherapy, 1976
- Factors influencing the activity of antimicrobial agents: an appraisal.1974
- Susceptibility of Bacteroides fragilis to Six Antibiotics Determined by Standardized Antimicrobial Disc Susceptibility TestingAntimicrobial Agents and Chemotherapy, 1973
- Effect of Several Components of Anaerobic Incubation on Antibiotic Susceptibility Test ResultsAntimicrobial Agents and Chemotherapy, 1972
- The Susceptibility of Bacteroides fragilis to 24 AntibioticsThe Journal of Infectious Diseases, 1972
- The effect of carbon dioxide on the sensitivity ofBacteroides fragilisto certain antibioticsin vitroJournal of Clinical Pathology, 1970
- CLINICAL EVALUATION OF ERYTHROMYCINArchives of Internal Medicine, 1954
- The Antibacterial Action of Erythromycin.Experimental Biology and Medicine, 1952