Serotonin uptake and release by biochemically characterized nerve endings isolated from rat brain by concomitant flotation and sedimentation centrifugation
- 1 June 1975
- journal article
- Published by Springer Nature in Journal of Neural Transmission
- Vol. 37 (2), 111-126
- https://doi.org/10.1007/bf01663628
Abstract
Crude synaptosomal preparations of rat brain were separated by concomitant flotation and sedimentation in a sucrose gradient into fractions containing myelin, nerve endings, and mitochondria, respectively. Two myelin-free nerve ending fractions were obtained which differ in the serotonin content and the activities of the enzymes 5-hydroxytryptophan decarboxylase, monoamine oxidase, choline acetylase, and acetylcholin-esterase. In glucose medium sodium ions stimulate serotonin uptake into the nerve endings while potassium ions in sucrose solution induce the release of this amine.Keywords
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