Inverse relationship of the durations of adjacent open and shut intervals for Cl and K channels
- 1 October 1985
- journal article
- Published by Springer Nature in Nature
- Vol. 317 (6038), 625-627
- https://doi.org/10.1038/317625a0
Abstract
Ion channels in cell membranes, whether voltage-dependent or activated by ligands, make repeated transitions among open and shut states during activity. Information about the number of states and the transitional pathways between them can be obtained from the durations of open and shut intervals, as transitions to states of different lifetimes result in intervals of different mean durations. If there is only one open conformation, or state, then the durations of open intervals would be independent of the durations of adjacent shut intervals. On the other hand, if a channel has two or more open states with different mean lifetimes, and if each open state is entered directly from a different shut state with a different mean lifetime, then the open intervals should be related to the adjacent shut intervals. We now report that the durations of adjacent open and shut intervals for both a C1 channel and a large conductance Ca-activated K channel in skeletal muscle are inversely related; shorter open intervals are adjacent to longer shut intervals. These findings indicate that two or more shut states make direct transitions to two or more open states, and suggest that the lifetimes of adjacent open and shut states are inversely related.Keywords
This publication has 14 references indexed in Scilit:
- Single chloride-selective channels active at resting membrane potentials in cultured rat skeletal muscleBiophysical Journal, 1985
- Calcium dependence of open and shut interval distributions from calcium‐activated potassium channels in cultured rat muscle.The Journal of Physiology, 1983
- Fitting and Statistical Analysis of Single-Channel RecordsPublished by Springer Nature ,1983
- Reconstitution in planar lipid bilayers of a Ca2+-dependent K+ channel from transverse tubule membranes isolated from rabbit skeletal muscle.Proceedings of the National Academy of Sciences, 1982
- Fluctuations in the microsecond time range of the current through single acetylcholine receptor ion channelsNature, 1981
- Single channel recordings of Ca2+-activated K+ currents in rat muscle cell cultureNature, 1981
- Improved patch-clamp techniques for high-resolution current recording from cells and cell-free membrane patchesPflügers Archiv - European Journal of Physiology, 1981
- Ca-dependent K channels with large unitary conductance in chromaffin cell membranesNature, 1981
- An analysis of the dose‐response relationship at voltage‐clamped frog neuromuscular junctions.The Journal of Physiology, 1978
- CONDUCTANCE FLUCTUATIONS AND IONIC PORES IN MEMBRANESAnnual Review of Biophysics and Bioengineering, 1977