Utilization of 1‐hydroxybenzotriazole in mixed anhydride coupling reactions
- 1 April 1985
- journal article
- research article
- Published by Wiley in International Journal of Peptide and Protein Research
- Vol. 25 (4), 408-413
- https://doi.org/10.1111/j.1399-3011.1985.tb02193.x
Abstract
The coupling of Boc-Val-OH to either H-Pro-OBzl or H-Pro-Gly-Val-Gly-OBzl by the mixed anhydride method leads to the formation of a urethane by-product in yields of 40–60%. This side reaction can be suppressed by the addition of HOBt to the reaction mixture before the amino component is added. This results in a substantially increased yield of the desired peptide.Keywords
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