QUANTITATIVE-ANALYSIS OF L-HYDROXYPROLINE IN HUMAN BIOLOGICAL-MATERIAL BY GAS-CHROMATOGRAPHY

Abstract

Quantitative amino acid analysis by gas chromatography was applied to the determination of 4-hydroxy-L-proline in human blood. After addition of a known amount of allo-4-hydroxy-D-proline, quantitative analysis of 4-hydroxy-L-proline can be performed by gas chromatographic separation of the amino acid enantiomers; since the standard and sample have identical chemical behavior, they respond identically during derivatization. The accuracy, reproducibility, and sample requirement of this procedure are more advantageous than those of hitherto existing methods. The method employs chiral stationary phases, which permit the simultaneous separation of amino acids and amino acid enantiomers.