Cis- and trans-Diamminedichloroplatinum(II)-mediated crosslinking of chromosomal nonhistone proteins to DNA in HeLa cells
- 24 April 1984
- journal article
- research article
- Published by American Chemical Society (ACS) in Biochemistry
- Vol. 23 (9), 1921-1926
- https://doi.org/10.1021/bi00304a005
Abstract
The cross-linking of chromosomal nonhistone proteins to DNA in isolated nuclei or intact HeLa cells exposed to different concentrations of [the antineoplastic drug] cis- and trans-diamminedichloroplatinum(II) (cis- and trans-DDP) for various time intervals was investigated by sodium dodecyl sulfate-polyacrylamide gel electrophoresis and immunochemical methods. Both the cis- and trans-DDP cross-linked significant numbers of chromosomal nonhistone proteins to the DNA. The quantity and the types of the cross-linked proteins depended on the time of incubation and on the concentrations of the drugs. Immunochemical techniques revealed that the 0.35-M NaCl insoluble and soluble chromosomal nonhistone proteins were cross-linked to the DNA by both isomers. Action of cis- and trans-DDP was reversed and/or blocked by thiourea or 2-mercaptoethanol. Pretreatment of isolated nuclei or chromatin with iodoacetamide or N-ethylmaleimide did not prevent the DNA-protein cross-linking.This publication has 8 references indexed in Scilit:
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