HORMONAL REQUIREMENTS FOR GROWTH AND DIFFERENTIATION OF OB17 PREADIPOCYTE CELLS-INVITRO

Abstract

The ob17 cell line is a clonal line established from epididymal fat pads of c57 BL/6J ob/ob mice. After conversion into adipose-like cells, ob17 presents both the morphological and biochemical properties of mature rodent fat cells. The adipose conversion process is best represented by a stochastic model in which a pool of stem cells (adipoblasts) gives rise to clusters of adipose cells and to additional stem cells that remain in the population. The role of the different factors involved in the adipose conversion process of ob17 cells is discussed, i.e. mitogenic factors, that enhance the number of committed cells (ACF or adipose conversion factor(s)), lipogenic factors, that enhance the expression of adipocyte enzyme markers (insulin) and adipogenic factors that are obligatory requirements for adipose conversion (triiodothyronine, growth hormone and other pituitary factors).