Radioimmunoassay of CSF for encephalitogenic basic protein: a diagnostic test for MS?
- 4 November 1972
- journal article
- Vol. 107 (9), 856-9
Abstract
Competitive inhibition of binding between radioiodine-labelled encephalitogenic basic protein from human myelin ((125)I-HEProt) and normal human alpha-2 macroglobulin and between (125)I-HEProt and rabbit antiHEProt serum was used to study concentrated cerebrospinal fluid (CSF) under "blind" control for cross-reactivity with HEProt. Samples of CSF from patients meeting the standard criteria for definite MS and possible MS, and from patients with optic neuritis and "other" diagnoses were studied. CSF from patients in all four groups was shown to have an inhibitor cross-reactive with HEProt when studied by the (125)I-HEProt/alpha-2 macroglobulin test, but the amount was significantly greater in the definite MS group than in the "other" group. Results of the two tests on CSF from MS patients correlated, suggesting that the tests were identifying the same inhibitor. It was concluded that CSF contains an inhibitor similar to HEProt and that the amount present in CSF could be a useful diagnostic marker of MS.This publication has 4 references indexed in Scilit:
- Binding of encephalitogenic basic protein by serum alpha-globulins.1970
- Allergic encephalomyelitis: A comparison of the encephalitogenic A1 protein from human and bovine brainArchives of Biochemistry and Biophysics, 1970
- Radioimmunoassay with gel filtration for detecting antibody to basic proteins of myelin.1968