Improved measurement of13C,31P J coupling constants in isotopically labeled RNA
- 3 April 1995
- journal article
- Published by Wiley in FEBS Letters
- Vol. 362 (2), 156-160
- https://doi.org/10.1016/0014-5793(95)00232-x
Abstract
3JCP coupling constants have been measured in 1 99% 13C, 15N labeled lead-dependent ribozyme, known as the leadzyme. These coupling constants were determined by analysis of the intensity of individual crosspeaks in a spin-echo difference constant time HSQC experiment. This procedure permits improved measurement of the 3JC2′P and 3JC4′P coupling constants in isotopically labeled RNA and yielded valuable information on the β and ϵ backbone torsion angles in the leadzymeKeywords
This publication has 25 references indexed in Scilit:
- Three-Dimensional Triple-Resonance 1H, 13C, 31P Experiment: Sequential Through-Bond Correlation of Ribose Protons and Intervening Phosphorus along the RNA Oligonucleotide BackboneJournal of the American Chemical Society, 1994
- Two-dimensional triple-resonance HCNCH experiment for direct correlation of ribose H1' and base H8, H6 protons in 13C,15N-labeled RNA oligonucleotidesJournal of the American Chemical Society, 1993
- Unambiguous resonance assignments in carbon-13, nitrogen-15-labeled nucleic acids by 3D triple-resonance NMRJournal of the American Chemical Society, 1993
- An Efficient Procedure for Assignment of the Proton, Carbon and Nitrogen Resonances in 13C/15N Labeled Nucleic AcidsJournal of Molecular Biology, 1993
- A constant-time 2D overbodenhausen experiment for inverse correlation of isotopically enriched speciesJournal of Magnetic Resonance (1969), 1992
- Three-dimensional heteronuclear NMR studies of RNANature, 1992
- Application of four-dimensional heteronuclear NMR to the structure determination of a uniformly 13C labeled RNAJournal of the American Chemical Society, 1992
- RNA structure and NMR spectroscopyQuarterly Reviews of Biophysics, 1991
- Rapid recording of 2D NMR spectra without phase cycling. Application to the study of hydrogen exchange in proteinsJournal of Magnetic Resonance (1969), 1989
- Accurate measurements of homonuclear HN-H.alpha. coupling constants in polypeptides using heteronuclear 2D NMR experimentsJournal of the American Chemical Society, 1989