MICRO METHOD FOR PERFORMING TITRATION AND NEUTRALIZATION TEST OF HOG-CHOLERA VIRUS USING ESTABLISHED PORCINE KIDNEY-CELL STRAIN

Abstract

Hog cholera (HC) virus and antibody were estimated by the exultation of Newcastle disease (END) method using microplates and CPK porcine kidney cells. To establish a technique for this method, studies were made on such basic conditions as the strain of Newcastle disease virus (NDV), the time of challenge with this virus and the concentration of serum in the culture fluid. There was little difference in the infective titer of HC virus estimated by the END method performed by the established technique and the same method by the conventional technique using swine testicle (ST) cells. There was a high correlation between the neutralizing antibody titer measured by the 2 techniques. The correlation coefficient was r = +0.948 in this case. The END micro-technique using CPK cells was simpler than and as reliable as the same method conducted by any conventional technique and was capable of testing many samples.