Measurement by Quantimet 720 of the proportion of actively growing cells in tissue cultures of human lymphocytes
- 1 January 1979
- journal article
- research article
- Published by Wiley in Journal of Microscopy
- Vol. 115 (1), 51-63
- https://doi.org/10.1111/j.1365-2818.1979.tb00151.x
Abstract
The size-distribution of normal human lymphocytes growing in tissue culture was measured with a Quantimet 720 image-analysing computer. The proportion of cells undergoing volumetric growth after phytohaemagglutinin (PHA) stimulation was derived from a simple mathematical model describing the growth patterns of the cells up to the first mitotic division. The approach may have general application in biology as a method for measuring the proportion of growing cells by microscopy.This publication has 14 references indexed in Scilit:
- Quantitation of lymphocyte proliferative response to allogeneic cells and phytohemagglutinin by flow cytofluorometryClinical Immunology and Immunopathology, 1976
- Quantitation of transformed lymphocytes by flow cytofluorimetry: I. Phytohemagglutinin responseClinical Immunology and Immunopathology, 1975
- STIMULATED LYMPHOCYTE CULTURESThe Journal of Experimental Medicine, 1974
- Studies on in vitro lymphocyte proliferation in cultures synchronized by the inhibition of DNA synthesis: II. Kinetics of the initiation of the proliferative responseExperimental Cell Research, 1970
- Modes of Growth in Mammalian CellsBiophysical Journal, 1969
- Cell Growth and DivisionBiophysical Journal, 1967
- Cell Growth and DivisionBiophysical Journal, 1967
- THE GROWTH AND MITOSIS OF HUMAN SMALL LYMPHOCYTES AFTER INCUBATION WITH A PHYTOHÆMAGGLUTININQuarterly Journal of Experimental Physiology and Cognate Medical Sciences, 1963
- Observations on the Proliferation of Human Leucocytes Cultured with PhytohaemagglutininBritish Journal of Haematology, 1963
- DNA synthesis and mitosis in cultures of human peripheral leukocytesExperimental Cell Research, 1962