Asymmetric and symmetric synaptic junctions in the dorsal lateral geniculate nucleus of cat and monkey

Abstract

We have investigated the applicability of traditional classifications of synaptic junctions in the dorsal lateral geniculate nucleus (DLGN) of the cat and monkey. Our principal sample is restricted to synapses made by the retinal terminal (round vesicles) in DLGN and to synapses made by flattened vesicle processes that were postsynaptic to the retinal terminal. We inspected consecutive thin sections through 250 synaptic junctions that showed clear synaptic clefts in every section. The thickness of the membrane and postsynaptic density (PSD) was measured on each section and an average thickness was computed for each synaptic junction. In both species the frequency distribution for these measurements forms an uninterrupted progression from the absence of a continuous PSD through the presence of a heavy density with most synaptic contacts falling in the midrange. Twenty-two of the round vesicle profiles and 40 of the flat vesicle profiles we studied had very modest densities (13-16 nm) and exhibited a continuous PSD on some sections, but only small puffs or a complete lack of density on others. We concluded that this group which constituted 25% of the synaptic contacts we studied could not be classified as asymmetric or symmetric. As a group the round vesicle synaptic junctions exhibited a heavier PSD than the flat vesicle contacts. The difference between the mean thickness in both species was statistically significant. However, we hesitate to describe the round vesicle synapses as asymmetric and the flat vesicle contacts as symmetric because such a large proportion of the former made synaptic contacts with a PSD thickness within the range of the latter.