Location of a gene specifying phosphopyruvate synthase activity on the genome of Escherichia coli , K12

Abstract

A gene which specifies the activity of phosphopyruvate synthase has been located on the genome of Escherichia coli. By interrupted mating of a variety of genetic donor (Hfr) and recipient (F$^{-}$) strains of Escherichia coli, K12, which carried alleles of the phosphopyruvate synthase (pps) gene, it was established that the pps gene is close to the aro-D marker. This was confirmed by phage-mediated transduction of these genes, which were shown to exhibit a high degree of linkage.