IMMUNOCHEMICAL INVESTIGATIONS OF HUMAN PITUITARY β‐LIPOTROPIN

Abstract
Antiserum to human β-lipotropin has been raised in rabbits and characterized by gel double diffusion, quantitative precipitin, microcomplement fixation and biological neutralization tests. A sensitive radioimmunoassay for human β-lipotropin has been developed. It was demonstrated that the antiserum shows specificity for this hormone. Human β-melanotropin and human β-endorphin showed only a minimal degree of cross-reaction, while ovine β-lipotropin showed a partial but significant degree of cross-reactivity.