PRESERVATION OF ACETYLCHOLINE IN HUMAN CEREBROSPINAL FLUID: A SIMPLE METHOD FOR CLINICAL USE

Abstract

A simple and effective method for preservation of acetylcholine in human cerebrospinal fluid has been described. The method depends on the use of acidification and prostigminization of cerebrospinal fluid. No detectable loss of acetylcholine for periods up to seven days or longer has been observed. The method has been satisfactorily used in clinical investigations on more than 200 cerebrospinal fluid samples from over 100 neurological and neurosurgical patients.